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Antibodies

VGAT, Monoclonal Antibody

Product Size(s)
100 ug
£500.00
131-011-SY
Academic Pricing
Please contact us for academic pricing.
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About this Product
SKU:
131-011-SY
Additional Names:
VGAT, VIAAT|VIAAT, SLC32A1
Application:
Electron Microscopy, FACS, IHC-Paraffin, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
PBS
Clone:
117G4
Clonality:
Monoclonal
Extra Details:
100 ug purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 ul H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Gene Details:
Slc32a1
Host:
Mouse
Immunogen:
Synthetic peptide corresponding to AA 75 to 87 from rat VGAT (UniProt Id: O35458)
Isotype:
IgG3
Protein Details:
O35458
Purification:
Affinity Purified
Reactivities:
Guinea Pig, Human, Mouse, Non-human Primate, Rat
Shipping Conditions:
Blue Ice
Specificity:
VGAT
Storage Conditions:
Please refer to datasheet
Supplier:
Synaptic Systems
Type:
Antibody: Monoclonal Antibody
Size:
100 ug
Synonyms:
GABA and glycine transporter;rGVAT;solute carrier family 32 (GABA vesicular transporter), member 1;Solute carrier family 32 member 1;vesicular GABA transporter;vesicular inhibitory amino acid transporter;vesicular inhibitory amino acid transporter-like;Vgat;Viaat
antibody dilution: 1 : 1000 sample: synaptic vesicle fraction of rat brain (LP2)
Double immunofluorescence labeling of a cultured hippocampal neuron (31 DIV) with anti-VGAT (dilution 1 :1 000) and anti-synapsin (cat. no. 106 002; dilution 1 : 20000) in combination with secondary Alexa-conjugated antibodies (Molecular Probes, Eugene, OR). A subset of synapses (synapsin; blue) is positive for VGAT (red). Image width: 80 um; Fixation: 4 % paraformaldeyde. Courtesy: G.J. Obermair and B.E. Flucher, Dept. of Physiology, University of Innsbruck.
Immunolabeling showing inhibitory synapses in the central nervous system. Immunoreactivity was revealed with carbocyanine 2, 3, and 5-conjugated secondary antibodies. Parvalbumin (PV) and choline-acetyltransferase (ChAT) were detected with rabbit and goat antibodies. Ac: commissura anterior, VP: ventral pallidum Rats were transcardially perfused with a fixative containing 4 % paraformaldehyde and 0.1 % glutaraldehyde in phosphate buffer (0.1M, pH 7.4). Courtesy: Tibor Harkany, Dept. of Medical Biochemistry & Biophysics, Karolinska Institute, Stockholm
Indirect immunostaining of PFA-fixed paraffin embedded mouse hippocampus section with anti-VGAT (dilution 1 : 200; green) and rabbit anti-VGluT 3 (cat. no. 135 203, dilution 1 : 200; red). Nuclei have been visualized by DAPI staining (blue).
Indirect immunostaining of PFA fixed paraffin embedded mouse brain section with mouse anti-VGAT (cat. no. 131 011, dilution 1 : 100; DAB). Nuclei have been visualized by haematoxylin staining (blue).