Recombinant lectins may offer a more reproducible alternative to their native counterparts, making them particularly suitable for Critical Quality Attribute (CQA) biotherapeutic manufacturing.
Lectin specificity for its carbohydrate moiety can be useful for purifying glycoproteins, such as fucosylated therapeutic antibodies, as the same purification method can be employed irrespective of the antibody's specificity.
RPL's can be used for biolayer inferometry (BLI) assays, to provide label free, high throughput real time glycoanalysis compared to HPLC.
| RPL Code |
Sugar specificity |
MW details |
|
RPL-αGal
|
Terminal α-linked Galactose & N-Acetylgalactosamine (GalNAc)
|
Monomer 14,161Da
(Dimer under physiological conditions)
|
|
RPL-Gal1
|
Terminal β-linked Galactose & N-Acetyllactosamine (LacNAc)
|
Monomer 14,066Da
(Dimer under physiological conditions)
|
|
RPL-Gal2
|
Terminal α-linked Galactose > N-Acetylgalactosamine (GalNAc)
|
Monomer 14,162Da
(Dimer under physiological conditions)
|
|
RPL-Gal3
|
Terminal α-linked Galactose
|
Monomer 14,081Da
(Dimer under physiological conditions)
|
|
RPL-Gal4
|
Terminal β-linked Galactose, N-Acetyllactosamine (LacNAc) & Lewis x (Lex)
|
Monomer 14,595Da
(Dimer under physiological conditions)
|
|
RPL-αMan
|
Fucose/Mannose: Lewis a (Lea), Lewis x (Lex) & terminal α-mannose
|
Monomer 13,130Da
(Dimer under physiological conditions)
|
|
RPL-Man2
|
Terminal α -mannose
|
Monomer 15,163Da
(Dimer under physiological conditions)
|
|
RPL-Sia1
|
Terminal α2-3-linked Sialic Acid (Neu5Ac) – on both N-linked and O-Linked Glycans
|
Monomer MW: 27,407.14 Da.
|
|
RPL-Sia2
|
Terminal α2-3-linked Sialic Acid (Neu5Ac) on O-Linked Glycan
|
Monomer MW: 40,725.17 Da.
|
|
RPL-Sia3
|
Terminal α-linked Neu5Ac
|
Monomer MW: 19,584.89 Da
|
|
RPL-Fuc1
|
α-linked Fucose
|
Monomer MW: 35,848.92 Da
|
|
RPL-Fuc2
|
α-linked Fucose
|
Monomer MW: 10,993.91 Da
|