PD-1

Programmed cell death protein 1 (PD-1), alternatively referred to as CD279, is an immunoglobulin superfamily member. It is expressed by activated T-cells and plays an important role in subsiding immune responses and promoting tolerance to self-antigens by suppressing T-cell activity. PD-1 has two natural ligands, known as PD-L1 (also known as CD274) and PD-L2 (CD273), that are mainly expressed by antigen presenting cells. Since over-expression of PD-L1 is common in many tumours, several monoclonal antibody therapeutics have been developed to block the PD-1/PD-L1 interaction. These include nivolumab (OPDIVO®) and pembrolizumab (Keytruda®), which have seen clinical success in treating melanoma.

PD-1 related antibodies from preferred suppliers:

• BE0310 - InVivoMAb anti-mouse VISTA - The 13F3(clone) monoclonal antibody reacts with mouse V-domain Ig suppressor of T cell activation (VISTA) also known as PD-1H and B7-H5.
• BE0273 - InVivoMAb anti-mouse PD-1 (CD279) - The 29F.1A12(clone) monoclonal antibody reacts with mouse PD-1 (programmed death-1) also known as CD279.
• BE0193 - InVivoMAb anti-human PD-1 (CD279) - The J110(clone) monoclonal antibody reacts with human PD-1 (programmed death-1) also known as CD279.
• BE0188 - InVivoMAb anti-human PD-1 (CD279) - The J116(clone) monoclonal antibody reacts with human PD-1 (programmed death-1) also known as CD279
• BE0146 - InVivoMAb anti-mouse PD-1 (CD279) - The RMP1-14(clone) monoclonal antibody reacts with mouse PD-1 (programmed death-1) also known as CD279.
• BE0033-2 - InVivoMAb anti-mouse PD-1 (CD279) - The J43 monoclonal antibody reacts with mouse PD-1 (programmed death-1) also known as CD279.

Below are more purified versions

for more information on the differences between InVivoMAb and InVivoPlus please click HERE!

• BP0310 - InVivoPlus anti-mouse VISTA - The 13F3(clone) monoclonal antibody reacts with mouse V-domain Ig suppressor of T cell activation (VISTA) also known as PD-1H and B7-H5.
• BP0273 - InVivoPlus anti-mouse PD-1 (CD279) - The 29F.1A12(clone) monoclonal antibody reacts with mouse PD-1 (programmed death-1) also known as CD279.
• BP0146 - InVivoPlus anti-mouse PD-1 (CD279) - The RMP1-14(clone) monoclonal antibody reacts with mouse PD-1 (programmed death-1) also known as CD279. 
• BP0033-2 - InVivoPlus anti-mouse PD-1 (CD279) - The J43(clone) monoclonal antibody reacts with mouse PD-1 (programmed death-1) also known as CD279.

PD-1 related proteins from preferred suppliers:

• PD1-R52H0 - Rabbit PD-1 / PDCD1 Protein, His Tag- is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # G1SUF0). This protein carries a human IgG1 Fc tag at the C-terminus. Fc Tag is also available.
• PD1-C5223 - Cynomolgus PD-1 / PDCD1 Protein, His Tag - Fc Tag is also available.
• PD1-R52H2 - Rat PD-1 / PDCD1 Protein, His Tag - Fc Tag is also available.
• PD1-M5228 -  Mouse PD-1 / PDCD1 Protein, His Tag - Fc Tag is also available.
• PD1-HC214 - Human PD-1 / PDCD1 Protein, Tag Free, Full Length - Twin-strep tag is also available.

PD-1 related Conjugated proteins:

• PD1-M82F4 - Biotinylated Mouse PD-1 / PDCD1, Fc Tag, Avi Tag (Avitag™) - Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labelled with biotin.

• PD1-M52A4 - Mouse PD-1 / PDCD1 Protein, mouse IgG2a Fc Tag, low endotoxin

• PD1-H82F2 - Biotinylated Human PD-1/PDCD1, His Tag & Fc Tag, Avi Tag (Avitag™) - This protein carries a human IgG1 Fc fragment at the C-terminus, followed by a polyhistidine tag. The Avi tag (Avitag™) is inserted in-between the Fc and His tags.

• PD1-H82E4 - Biotinylated Human PD-1 / PDCD1 Protein, Avitag™,His Tag (recommended for biopanning) - This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.

• PD1-HP2F2 - PE-Labelled Human PD-1 / PDCD1 Protein, Fc Tag, His Tag - This protein carries a human IgG1 Fc tag at the C-terminus, followed by a polyhistidine tag.

PD-1 related ELISA kits from preferred suppliers:

• ARG81342 - Mouse CD279 / PD-1 ELISA Kit - Enzyme Immunoassay kit for the quantification of mouse CD279 / PD-1 in serum, plasma and cell culture supernatant.

• ARG81360 - Human CD279 / PD-1 ELISA Kit - Enzyme Immunoassay kit for the quantification of human CD279 / PD-1 in serum, plasma and cell culture supernatant.

• ARG81580 - Human CD273 / PD-L2 ELISA Kit - Enzyme Immunoassay kit for the quantification of human CD273 / PD-L2 in serum, plasma (heparin, EDTA) and cell culture supernatants.

PD-1 related Cell Lines from preferred supplier:

- M00552 - CHO-K1/mPD-1 Stable Cell Line - (Academic Discount Available) High protein expression at the cell surface validated by FACS. Stable cell lines for increased reliability and reproducibility (over 15 passages). Ready to use hot targets, saving 3-5 months of drug discovery program. For Binding Assay.