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Antibodies

m6A, Polyclonal Antibody

Product Size(s)
50 ug
£506.00
202-003-SY
Academic Pricing
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About this Product
SKU:
202-003-SY
Additional Names:
M6A|N6-methyladenosine, Adenosine
Application:
ELISA, IHC-Paraffin, Immunocytochemistry, Immunoprecipitation, Western Blot
Buffer:
PBS
Clonality:
Polyclonal
Extra Details:
50 ug specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 ul H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Host:
Rabbit
Immunogen:
N6-methyladenosine fused to BSA
Purification:
Affinity Purified
Reactivities:
Bacteria, Human, Mouse, Other Species, Rat
Shipping Conditions:
Blue Ice
Specificity:
m6A
Storage Conditions:
Please refer to datasheet
Supplier:
Synaptic Systems
Type:
Antibody: Polyclonal Antibody
Size:
50 ug
100 ng of genomic DNA from Dam + and Dam - E.coli strains have been spotted and probed with rabbit anti-m6A (dilution 1 : 1000)
A: ELISA quantification of different m6A amounts. Rabbit anti-m6A was used as capture antibody and biotinylated oligonucleotides served as antigens. Oligos were composed of 24 nucleotides including five N6-methyl-2-deoxyadenosines (m6A oligo BT) or deoxyadenosines (negative control oligo BT) and were biotinylated at their 3'-ends. Streptavidin-conjugated HRP was used as detector. B: Release of antibody-bound m6A. Rabbit anti-m6A was used as capture antibody and was saturated with biotinylated m6A oligonucleotides. Different amounts of free m6A (Sigma) were added to compete with bound oligos. Streptavidin-conjugated HRP was used as detector.