ImmPACT[R] DAB EqV Peroxidase (HRP) Substrate Citation Available Product Size(s) 100ml £188.00 SK-4103-100 Available for immediate dispatch Add to order Added to order 400ml £604.00 SK-4103-400 Add to order Added to order Academic Pricing Please contact us for academic pricing. Contact Us About this Product SKU: SK-4103 Additional Names: Enzyme Substrates [Enzyme Substrates ] Precipitating HRP Substrate, Horseradish Peroxidase substrate Application: Immunohistochemistry, IHC-Frozen, IHC-Paraffin, Immunocytochemistry, ELISPOT, FLUOROSPOT Extra Details: DAB (3, 3 -diaminobenzidine) HRP substrate produces a dark brown reaction product and can be used for both immunohistochemical and blotting applications. DAB chromogen is effective as a single label or as a second color for multiple antigen labeling. Because of its heat-resistance, DAB can be used in IHC/ ISH double labeling applications. With the aid of imaging systems and software, the spectral profile of DAB can be distinguished from our other proprietary enzyme substrates in applications where antigens are co-localized. Sections stained with DAB can also be viewed by darkfield and electron microscopy. Slides developed with DAB can be dehydrated, cleared, and permanently mounted. The DAB reaction product can be intensified with DAB Enhancing Solution (H-2200).ImmPACT[TM] DAB EqV (equal volume) consists of 200 ml each of Reagent 1 and Reagent 2 which when mixed together in equal volumes form the working solution. ImmPACT[TM] DAB EqV produces a dark brown reaction product that is 3 to 5 times more sensitive than the original DAB substrate. The working solution is stable for several days when stored at 4 °C. This substrate can be used for both manual and automated staining methods. ImmPACT[TM] DAB EqV can be used for blot detection on nylon and PVDF membranes but is not recommended for nitrocellulose membranes.Features:3 - 5 times more sensitive than the original DAB substrateOptimized for IHC/ICC/ISHHeat StablePermanent mountingIdeal for single and multiple labelingCan be used for blot detection on nylon and PVDF membranes but is not recommended for nitrocellulose membranesWorking solution is stable for several days when stored at 4 °COne year expiry dateConsists of 200 ml each of Reagent 1 and Reagent 2 which when mixed together in equal volumes form the working solution Shipping Conditions: Ambient Storage Conditions: 2-8 [o]C Supplier: Vector Laboratories Manufacturer's Data Sheet:immpact-dab-eqv-hrp-substrate SK-4103-100_ImmPACT_DAB_EqV_HRP_Substrate.jpg SK-4103-400_ImmPact_DAB_EqV_HRP_Substrate.jpg Need Help? View product citations for antibodies SK-4103 on CiteAb