Monoclonal antibodies
Development of monoclonal antibodies (MAbs) using HybriFree Technology is attractive for generation of immunomolecules from species other than mice. There are several technologies described for the development of recombinant monoclonal antibodies including phage display, single B-cell cloning, proteomics, NGS (Next Generation Sequencing) based etc. However, these all have drawbacks: using bacterial or yeast expression system in phage display, selected MAbs may not be functional; problems with robustness (single B-cell manipulations and/or sterile cultivation of B-cells) may occur; or expensive devices or patented technologies are needed.
Polyclonal antibodies
Our partner lab provides a full service production of polyclonal antibodies in rabbit or chicken hosts. For immunization, KLH (or other carrier) conjugated peptides, recombinant proteins and other available immunogenes could be used. We recommend using recombinant proteins for immunization due to the stronger immune response and larger number of epitopes available when compared with peptide immunization.
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