Concanavalin A (Con A), Biotinylated Citation Available Product Size(s) 5mg £132.00 B-1005-5 Add to order Added to order Academic Pricing Please contact us for academic pricing. Contact Us About this Product SKU: B-1005 Additional Names: Lectins & Glycobiology Reagents [Lectins ], Concanavalin enisformis (Jack Bean), alpha-methyl mannoside/ alpha- methylglucoside binding lectin Application: Immunohistochemistry, IHC-Frozen, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, ELISPOT Conjugate: Biotin Extra Details: Con A recognizes α-linked mannose present as part of a core oligosaccharide in many serum and membrane glycoproteins. At neutral and alkaline pH, Con A exists as a tetramer of four identical subunits; below pH 5.6, Con A dissociates into active dimers of 52kDa. Acetylation, succinylation, or other derivatizations can also produce stable forms with dimeric structures. (See succinylated Con A). Nicks in the sequence are often present in the purest preparations due to hydrolytic damage within the seeds.Con A requires calcium or manganese ions at each of its four saccharide binding sites. Although these divalent metal ions are bound tightly to the polypeptide structure, buffers which can bind calcium (such as phosphate) generally should be avoided in diluting Con A, since a gradual loss in activity may occur.Biotinylated Concanavalin A has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium sodium azide.This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative. Inhibiting/Eluting Sugar: mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside Shipping Conditions: Ambient Storage Conditions: 2-8 [o]C; Store frozen for long term storage Supplier: Vector Laboratories Manufacturer's Data Sheet:biotinylated-concanavalin-a-con-a B-1005-5_Concanavalin_A_Con-A_Biotinylated.jpg Need Help? Related Products alpha-methylmannoside 5ml From View Product alpha-methylglucoside 5ml From View Product Concanavalin A (Con A), Agarose bound 10ml, 100ml From View Product View product citations for antibodies B-1005 on CiteAb