Amyloid Beta Peptide 1-42 (HFIP treated) Monomers Citation Available Product Size(s) 100 ug £244.00 SPR-485-100UG Add to order Added to order 500 ug £406.00 SPR-485-500UG Add to order Added to order 1 mg £600.00 SPR-485-1MG Add to order Added to order Academic Pricing Please contact us for academic pricing. Contact Us About this Product SKU: SPR-485 Additional Names: Abeta Protein, Abeta peptide, Amyloid beta peptide, Beta amyloid peptide, amyloid beta precursor protein peptide, APP Application: Cell-based/Functional Assay, Western Blot CE/IVD: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only. Extra Details: Our amyloid beta peptide 1-42 (AB Beta42) is produced synthetically and treated with 1,1,1,3,3,3-Hexafluoro-2-propanol (HFIP) prior to drying which breaks down pre-formed fibrils and monomerizes the peptide, as previously published (1,2). Upon resuspension in DMSO/dH2O, our AB Beta42 presents as a monomeric peptide without fibrils when observed under TEM, AFM and on a Western Blot with an anti-amyloid beta antibody. In contrast to AB42 oligomer and fibril constructs, our AB Beta42 monomers were not toxic to primary rat cortical neurons. In the brain, amyloid beta peptide (AB Beta) is generated by protease cleavage of amyloid precursor protein (APP), which aggregates into oligomers, protofibrils, fibrils and ultimately plaques in neurodegenerative diseases. The accumulation of AB Beta plaques in the brain is considered a hallmark of Alzheimer's disease (AD), and most of the drugs tested for AD in the past 20 years have targeted amyloid beta accumulation (3). Soluble AB Beta oligomers isolated from the brains of AD patients or those generated in vitro potently impaired synapse structure and function (4). AB Beta oligomers generated in vitro were toxic to PC12 cells (2) and SH-SY5Y cells (5). AB Beta was demonstrated to interact with tauopathies to affect neurodegeneration in AD patients (6) and accumulations of AB Beta were shown to be associated with lower survival rates in Parkinson's disease patients with dementia (7). Molecular Weight: 4.5 kDa Purity: >98% Sequence: DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA Shipping Conditions: Blue Ice Storage Conditions: -70[o]C Supplier: StressMarq Biosciences Type: Proteins, Peptides, Small Molecules & Other Biomolecules: Synthetic Manufacturer's Data Sheet: Epitope Tag: Untagged Western blot of amyloid beta 1-42 monomers (SPR-485, left), oligomers (SPR-488, middle) and fibrils (SPR-487, right) using anti-amyloid beta 6E10 antibody. Amyloid beta constructs at 160 pmol were run on 4-12% Bis-Tris SDS-PAGE, transferred to nitrocellulose in the presence of 0.02% v/v Tween-20, and blotted with 1:1000 mouse 6E10 primary antibody (Biolegend). Oligomers observed under TEM/AFM show distinct dimer/trimer bands as well as a signal from ~37-75 kDa (middle). Fibrils observed under TEM/AFM show a signal greater than 100 kDa and a distinct signal in the stacking gel (right). Amyloid beta 1-42 oligomers (SPR-488) and fibrils (SPR-487) show a dose-dependent toxicity to primary rat cortical neurons, but not monomers (SPR-485). Survival of rat primary cortical neurons 14 days after treatment with different concentrations of (A) monomers, (B) oligomers or (C) fibrils quantified by MAP2 positive neurons and expressed as a percentage of control. Fibrils and respective vehicle controls were initially sonicated in a Bioruptor. Test conditions were run in the same plate as untreated control and vehicle controls, which consisted of buffer without amyloid beta 1-42 protein. Data expressed as mean +/- s.e.m. (n=6). A global analysis of the data was performed using a one-way ANOVA followed by Dunnett's test; ** p<0.01 stats vs control; ## p<0.01, #### p<0.0001 stats vs vehicle control. represents untreated control condition. TEM of amyloid beta 1-42 monomers (SPR-485, left), oligomers (SPR-488, middle) and fibrils (SPR-487, right). Negative stain transmission electron microscopy images acquired at 80 Kv on carbon coated 400 mesh copper grids using phosphotungstic acid and uranyl acetate stain. Scale bar = 100 nm. Need Help? View product citations for proteins SPR-485 on CiteAb