Amyloid Beta 1-42 Oligomers Citation Available Product Size(s) 100 ug £844.00 SPR-488-100UG Add to order Added to order 2 x 100 ug £1280.00 SPR-488-2X100UG Add to order Added to order 5 x 100 ug £2468.00 SPR-488-5X100UG Add to order Added to order Academic Pricing Please contact us for academic pricing. Contact Us About this Product SKU: SPR-488 Additional Names: Abeta Oligomers, Abeta peptide, Amyloid beta peptide oligomers, Beta amyloid peptide oligomers, amyloid beta precursor protein peptide oligomers, APP Application: Cell-based/Functional Assay, Western Blot CE/IVD: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only. Extra Details: Our Amyloid Beta 1-42 (AB Beta42) Oligomers are generated from Amyloid Beta Peptide 1-42 pre-treated with 1,1,1,3,3,3-Hexafluoro-2-propanol (HFIP) as previously published (1,2). Our AB Beta42 oligomers present as globular oligomers when observed under TEM and AFM, and have a unique dimer/trimer and oligomer signal on a Western Blot with an anti-amyloid beta antibody. Our AB Beta42 oligomers were also demonstrated to be toxic to primary rat cortical neurons in a dose-dependent manner. In the brain, amyloid beta peptide (AB Beta) is generated by protease cleavage of amyloid precursor protein (APP), which aggregates into oligomers, protofibrils, fibrils and ultimately plaques in neurodegenerative diseases. The accumulation of AB Beta plaques in the brain is considered a hallmark of Alzheimer's disease (AD), and most of the drugs tested for AD in the past 20 years have targeted amyloid beta accumulation (3). Soluble AB Beta oligomers isolated from the brains of AD patients or those generated in vitro potently impaired synapse structure and function (4). AB Beta oligomers generated in vitro were toxic to PC12 cells (5) and SH-SY5Y cells (6). AB Beta was demonstrated to interact with tauopathies to affect neurodegeneration in AD patients (7) and accumulations of AB Beta were shown to be associated with lower survival rates in Parkinson's disease patients with dementia (8). Molecular Weight: 4.5 kDa Purity: >95% Sequence: DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA Shipping Conditions: Dry Ice Storage Conditions: -70[o]C Supplier: StressMarq Biosciences Type: Proteins, Peptides, Small Molecules & Other Biomolecules: Synthetic Manufacturer's Data Sheet: Epitope Tag: Untagged TEM of amyloid beta 1-42 oligomers (SPR-488). Negative stain transmission electron microscopy images acquired at 80 Kv on carbon coated 400 mesh copper grids using phosphotungstic acid and uranyl acetate stain. Scale bar = 100 nm. AFM of amyloid beta 1-42 oligomers (SPR-488). Atomic force microscopy analysis of 1.0 mg/mL samples diluted to 0.1 mg/mL in dH2O, mounted on freshly cleaved mica, washed, dried and analyzed with tapping mode. Representative images are 2.5 x 2.5 um x-y with a z-range of 10 nm. Western blot of amyloid beta 1-42 monomers (SPR-485, left), oligomers (SPR-488, middle) and fibrils (SPR-487, right) using anti-amyloid beta 6E10 antibody. Amyloid beta constructs at 160 pmol were run on 4-12% Bis-Tris SDS-PAGE, transferred to nitrocellulose in the presence of 0.02% v/v Tween-20, and blotted with 1:1000 mouse 6E10 primary antibody (Biolegend). Oligomers observed under TEM/AFM show distinct dimer/trimer bands as well as a signal from ~37-75 kDa (middle). Fibrils observed under TEM/AFM show a signal greater than 100 kDa and a distinct signal in the stacking gel (right). Amyloid beta 1-42 oligomers (SPR-488) and fibrils (SPR-487) show a dose-dependent toxicity to primary rat cortical neurons, but not monomers (SPR-485). Survival of rat primary cortical neurons 14 days after treatment with different concentrations of (A) monomers, (B) oligomers or (C) fibrils quantified by MAP2 positive neurons and expressed as a percentage of control. Fibrils and respective vehicle controls were initially sonicated in a Bioruptor. Test conditions were run in the same plate as untreated control and vehicle controls, which consisted of buffer without amyloid beta 1-42 protein. Data expressed as mean +/- s.e.m. (n=6). A global analysis of the data was performed using a one-way ANOVA followed by Dunnett's test; ** p<0.01 stats vs control; ## p<0.01, #### p<0.0001 stats vs vehicle control. represents untreated control condition. Need Help? View product citations for proteins SPR-488 on CiteAb