AlphaThera

oYo-Link ® exhibits differences in its ability to covalently photo-crosslink various Immunoglobulins (Ig). This variability exists both between antibodies from different species and between different antibody subclasses from the same species. The table below gives an overview of the cross-linking efficiency of oYo-Link to different Ig species and subclasses.

Note: While crosslinking efficiency is usually consistent for antibodies from a specific species/host, some exceptions exist. Therefore, customers are encouraged to check the labeling efficiency of their antibody if labeling efficiency is critical for an experiment.

oYo-Link® products are compatible with all common buffers, including those containing storage proteins (e.g. BSA) and Tris. As a result, you will likely not need to dialyze or filter your antibody prior to labeling. oYo-Link also works across a wide range of pH values. The high affinity interaction between oYo-Link and IgG even enables antibodies to be labeled directly in serum or hybridoma supernatant, and antibody concentrations can be as low as 50 µg/mL.