Aleuria Aurantia Lectin (AAL), Agarose bound Citation Available Product Size(s) 2ml £342.00 AL-1393-2 Add to order Added to order Academic Pricing Please contact us for academic pricing. Contact Us About this Product SKU: AL-1393 Additional Names: Lectins & Glycobiology Reagents [Affinity Binding Matrices ], Mushroom Lectin, Fucose(alpha 1;6)N-Acetylglucosamine or Fucose (1;3)N-Acetyllactosamine binding lectin Application: Purification Conjugate: Agarose Extra Details: This lectin is a dimer of two identical subunits of about 36kDa each. Unlike Ulex europaeus and Lotus tetragonolobus lectins which prefer (α -1,2) linked fucose residues, Aleuria aurantia lectin binds preferentially to fucose linked (α -1,6) to N-acetylglucosamine or to fucose linked (α -1,3) to N-acetyllactosamine related structures. AAL also reversibly binds fucose attached to nucleic acids Agarose bound* Aleuria Aurantia lectin is prepared using our affinity-purified lectins. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2x107 daltons are used as the solid-phase matrix to which the lectins are covalently coupled. The attachment of the lectins to the beads is carefully controlled to preserve lectin activity and minimize conformational changes of the bound lectins that might result in nonspecific ionic or hydrophobic interactions. The technique we have developed to couple lectins to agarose beads inserts a hydrophilic spacer arm between the lectin and the matrix. This coupling method provides several advantages over the traditional cyanogen bromide procedure: Maximum carbohydrate binding activity of the coupled lectins is retained Linkage is stable over a range of pH values Conjugated proteins are not leached off the beads by Tris or other routinely used buffers No residual charges are present after conjugation. This minimizes non-specific binding to the matrix. Our agarose bound lectins are supplied at a constant concentration of lectin per ml of settled beads. The concentration for each lectin is selected to achieve the highest glycoconjugate binding capacity per mg of lectin present in the beads. Each lot is tested for its binding capacity using glycoproteins known to bind the lectin. This provides a guideline for the user and assures the quality of our agarose bound lectins. Inhibiting/Eluting Sugar: 100 mM L-fucose *2 mg lectin/ml gel Purification: Affinity Purified Research Area: Glycobiology Shipping Conditions: Ambient Storage Conditions: 2-8 [o]C DO NOT FREEZE Supplier: Vector Laboratories Manufacturer's Data Sheet:agarose-bound-aleuria-aurantia-lectin-aal AL-1393-2_Aleuria_Aurantia_Lectin_AAL_Agarose.jpg Need Help? View product citations for antibodies AL-1393 on CiteAb